Lab Protocols & Bioinformatics

PM-OICR TGL has developed optimized lab protocols for FFPE RNA-Seq, Exome and Methylation profiling. We use automated custom pipelines that integrate with Seqware and the MISO LIMS system. Our pipeline overviews are detailed below. For detailed inquires and replicating informatic pipelines please contact dax [dot] tortiatoicr [dot] on [dot] ca.

text for grant applications

We have summerized PM-OICR TGL's protocols, informatics, workflows, and lab overview into a document for easy "copy and paste" into grant applications, proposals and clinical research protocols. Please see our Manual for Grant Applications, Proposals and Clinical Research Protocols.

LAB PROTOCOLS

Illumina TruSeq Total RNA FFPE Protocol

Agilent XT Human FFPE Exome Protocol (V6 + COSMIC or V5+UTR)

KAPA Library Quantification Protocol

Qubit Assay for Nucleic Acid Quantification

Qiagen AllPrep DNA/RNA FFPE Extraction Protocol

BIOINFORMATIC PIPELINES

PM-OICR TGL Exome Pipeline V1.0

PM-OICR TGL Methyl Pipeline V1.0

PM-OICR TGL RNA Pipeline V1.0

PM-OICR TGL Github

Contact the PM-OICR Translational Genomics Laboratory

Dax Torti

Project Manager
PM-OICR Translational Genomics Lab

Email: dax [dot] tortiatoicr [dot] on [dot] ca

Phone: 647-260-7938

Ontario Institute for Cancer Research (OICR)
MaRS Centre
661 University Avenue
Toronto, Ontario M5G 0A3
Canada