SeqControl

About

SeqControl is a set of Perl and R scripts designed to assess the quality of sequencing data using multiple quality metrics. The pipeline takes one or more sorted BAM files as input, and outputs multiple files generated with open-source software that describe different aspects of the data. This data can then be summarized into a single fi le. The SeqControl quality metric vales from various BAMs can also be used to create predictive models to estimate data quality.
Figure: The fraction of yes votes (confidence that six lanes will achieve 50× coverage) received by each single-lane input shows separation between true positive and true negative observations (black vs. gray bars, respectively). Covariates representing metric values for single-lane inputs show that multiple metrics influence the number of yes votes received.

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Version 1.0.3
(40.2 KB)
Sample Files
(134.7 MB)
Preseq Testing Results
(16.31 MB)